If no recent changes made and things were working correctly, I might lean toward checking some less complex things!
The connections from tow to trailer are out in the weather as well as exposed to all kinds of potential damage, so I might look them over as a reasonably simple start.
When you have the tow vehicle running at highway type speed, do you get a full 13+ voltage at the battery posts? For current to run in to the batteries, we have to have a voltage somewhat higher than the normal charge we expect from a fully charged battery.
Current flows from higher to lower voltage, so if the difference in that "hill" drops to much, less and less current will flow.
One way to reduce that current flow is if we get some form of resistance in the path from the engine alternator to the toad batteries.
Slipping fan belt on alternator? Corrosion or poor contact at numerous places along the path? Even something as simple as corrosion on a connection.
We get a lot of posts about trailers that have electrical connections down under the frame at the front where we have to look up from under near the propane tanks.
If you have that setup, it seems to be a terrible plan to put that many breakers, etc. almost directly in any water from the road, so they tend to be a corrosion problem!
Perhaps try comparing the voltage of the tow vehicle battery to the toad battery as the engine is running. If there is a good bit of difference in the two, find where there is too much resistance in the line between the two!